Bengt Fadeel and Lucian Farcal
WP6 works on the application of in vitro methods for the assessment of nanomaterial (NM) immunotoxicity and genotoxicity. The hypothesis is that refined in vitro assays can replace in vivo testing of NMs. To achieve this goal, WP6 partners selected and refined the SOPs for the in vitro methods to be used within the project.
The final goal is to select and adapt a set of in vitro assays for High-Throughput Screening (HTS). In addition, WP6 partners will also deliver samples of NM-exposed cells to WP10 for further analysis. The data generated will ultimately be used to develop the Nanosafety Classifier.
A first set of experimental data on immunotoxicity and genotoxicity of CuO NMs with different surface properties were recently generated in WP6. The preliminary results suggested that the toxicity of CuO NMs is dependent upon the surface functional group.
The cytotoxicity effects were confirmed in different cellular models (i.e. macrophages vs. lung cells) and were also correlated with the level of intracellular uptake for some cell types. Further experimental work focuses on the elucidation of toxicity effects of the entire panel of NMs in NANOSOLUTIONS using various in vitro methodologies that may have a good potential to be adapted for HTS.